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In light of the data generated in this study, a more complete pharmacokinetic investigation designed to quantify the magnitude and duration of increase in GSH is warranted

HCT116 Flp-in cells (seeded in 10 cm plates, 30% confluency) were co-transfected with 9 g of donor plasmid and 3 g of sgRNA-Cas9 plasmid, and 24 l Turbofect (Thermo Scientific, R0533) in 2 ml Opti-MEM (Thermo Scientific, 11058-021)

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In many protocols vitamin C is administered together with glutathione because it helps stabilize antioxidant activity and may enhance the brightening effect