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The study reported that patients treated with a combination of thiopurine and allopurinol were more likely to achieve the desired 6-TGN levels than those receiving the split-dose regimen ( Other Factors Influencing Thiopurine Treatment Outcome Before prescribing thiopurines to the patients, several factors should be considered, as they can affect the outcome of thiopurine therapy

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A single colony from agar plate was cultured overnight in 10 ml of LB medium containing 50 g ml 1 kanamycin at 37 C and 160 rpm