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Using an ERE-luciferase mouse, a reporter mouse that allows monitoring of ER transcriptional activity in a spatiotemporal manner, it was demonstrated that estrogen signaling is locally activated in keratinocytes in vitro (with 1 nM E2) and in vivo (with 10 nM E2)

* P 110 13 genome copies/mL, Brain VTA) was injected using a glass micropipette connected to an ultra-micro injection pump (Nanoliter 2010, World Precision Instruments) with a flow rate of 70 nL/min

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